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Image Search Results
Journal: Scientific Reports
Article Title: Microtubule stabilising peptides rescue tau phenotypes in-vivo
doi: 10.1038/srep38224
Figure Lengend Snippet: For each phospho-tau antigen, intensity of signal (pixels/mm ) was normalised to total tau levels. There was no significant change in the levels of the phospho-tau epitopes detected by AT180 ( a ), AT8 ( b ), and PHF-1 ( c ) after treatment with 5 μg/ml SAL (green bars) and 10 μg/ml SAL (dark green bars) SAL. Total dtau levels were not altered by SAL treatment ( d ). Representative blots are shown (a-d). All lanes were run on the same gel. Data were analysed by one-way ANOVA with Bonferroni’s correction. Error bars represent mean ± S.E.M; n = htau 0N3R (5), 5 μg/ml SAL (5), 10 μg/ml SAL (5).
Article Snippet: Blots were probed with the following primary antibodies: anti-human tau (1:15,000, Dako, UK), anti-dtau (1:500, gifted by Prof. St. Johnston, University of Cambridge, UK), anti-actin (1:5000, Abcam, UK) and anti-phospho-tau: PHF-1 (1:2000, gifted by Dr. Peter Davies, Albert Einstein College of Medicine; Bronx, NY),
Techniques: